2XYQ,5YNQ,6WVN


Conserved Protein Domain Family
capping_2-OMTase_Nidovirales
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cd20762: capping_2-OMTase_Nidovirales 
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of nidovirales
Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Nidovirales viruses, which comprise a family of ss(+)RNA viruses, cap their mRNAs. For one member, coronavirus, the 2'OMTase activity is located in the non-structural protein 16 (Nsp16). For others, the 2'OMTase activity may be located in replicase polyprotein 1ab.
Links
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Statistics
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PSSM-Id: 467737
Aligned: 26 rows
Threshold Bit Score: 169.803
Created: 7-Oct-2019
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
Download Cn3D
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:SAM binding site [chemical binding site]
Evidence:
  • Structure:6WVN: SARS-CoV-2 2'-O-methyltransferase binds S-adenosylmethionine; contacts at 4A
  • Structure:2XYQ: SARS-coronavirus RNA cap 2'-O-methyltransferase NSP16 with bound SAM; contacts at 4A
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1         #   #                           ###              ##                       ###   
2XYQ_A         43 NVAKYTQLCQYLNTLtlav----pynMRVIHFGAGsdk--------gvaPGTAVLRQwlp------tgTLLVDSDLNdf- 103  SARS coronavirus
5YNQ_A         43 NIAKYMQLCQYLNTCtlav----panMRVIHFGAGsdk--------giaPGTSVLRQwlp------tdAIIIDNDLNef- 103  Human betacor...
6WVN_A         46 NVAKYTQLCQYLNTLtlav----pynMRVIHFGAGsdk--------gvaPGTAVLRQwlp------tgTLLVDSDLNdf- 106  Severe acute ...
YP_803213    6651 NIQKYDQMCDLFAIDlki-----pvkGHIHHLGNAgnk---------ysPGDVVLRQyf-------dqAHLTSYDLRev- 6708 White bream v...
P0C6V7       6630 NFAKYTQICTFIQDHvkv-----arnALVWHLGAAgvd--------gcsPGDIVLSSff-------keCLVYSWDIKdy- 6688 Berne virus
AVM87336     6613 NIHKYDQMCAFFDKHlti-----ptnGHVHHLGHAgqv---------ysPGDIVLKSyf-------tkARLTSYDLRdc- 6670 Chinook salmo...
AVM87568     2856 CYHKYHELMDFVARRlslpkkragitPRVLNFGARsegcgaqgsnaiakRWPEVDLY-----------SYDINQDYNtp- 2923 Hainan hebius...
YP_009505581 6955 NIHKFDQMCDFFATElki-----ptkGHVHHLGNAgnv---------ysPGDIVLKQyf-------nqAIISGYDLRdv- 7012 Fathead minno...
QEG08237     2473 CFLKYTQLLQYLNFStttpn-pmhnkLQVLNCGAAdi----------ngHSPGSDAIlaffrpdtpnaPNITLHDLDcnl 2541 Pacific salmo...
P0C6V8       6506 NFAKYTQICTFIQEHvkv-----arnSLIWHVGAAgid--------gcsPGDIVLSSff-------keCLVYSWDVKdy- 6564 Breda virus s...

Feature 1                     ##              ###                #                                
2XYQ_A        104 --vsDADSTLIGDCatvhtankwDLIISDMYDprtkhvtkendskeGFFTYLCGFIKqkl---alGGSIAVKITEHsw-n 177  SARS coronavirus
5YNQ_A        104 --vsDADITLFGDCvtvrvgqqvDLVISDMYDpttknvtgsneskaLFFTYLCNLINnnl---alGGSVAIKITEHsw-s 177  Human betacor...
6WVN_A        107 --vsDADSTLIGDCatvhtankwDLIISDMYDpktknvtkendskeGFFTYICGFIQqkl---alGGSVAIKITEHsw-n 180  Severe acute ...
YP_803213    6709 --vsDIPVLHPNDEwk------aHFILSDVYApdtd-------ftsLALEYMQNHLRl-------GGSIMWKMTETs--i 6764 White bream v...
P0C6V7       6689 ---sTLLDKHSYDCnf-----rpNLIVSDIYNvss--------nvsEVLDDCVHRLAl-------GGTIVFKTTESsrpd 6745 Berne virus
AVM87336     6671 --isTSETAHPDDNwr------aHFILSDAYCpntd-------ftsLCKDYMTKHLRl-------GGSMLWKMTETc--n 6726 Chinook salmo...
AVM87568     2924 ---gTKLLEFPTDEf--------DLIISDAWAysi--------dltEDLEALVSTLSf-------GGSMLVKITRTs--k 2975 Hainan hebius...
YP_009505581 7013 --itSSELKLPTDQwk------cHLLISDVYEkltd-------ftdLALDYITNHLHl-------GGSIIWKMTETs--t 7068 Fathead minno...
QEG08237     2542 ftsaAAVSFHIAIEqfsp-gisyDLIISDIYNs-------------DDLNYFTSLERiiksnlsfGGTFVIKMTETry-s 2606 Pacific salmo...
P0C6V8       6565 ---dTLLEKHNYDCnf-----rpNLIVSDVYNvss--------nvsEVLEDCVHRLAl-------GGTIIFKTTESsrpd 6621 Breda virus s...

Feature 1                                                                       
2XYQ_A        178 ADLYKLMGhFSWWTAFVTNvnaSSSEAFLIGAnyl--gkpKEQIDGyt----mHANYIFWRN 233  SARS coronavirus
5YNQ_A        178 VELYELMGkFAWWTVFCTNanaSSSEGFLLGInyl--gtiKENIDGga----mHANYIFWRN 233  Human betacoronavirus 2c EMC/2012
6WVN_A        181 ADLYKLMGhFAWWTAFVTNvnaSSSEAFLIGCnyl--gkpREQIDGyv----mHANYIFWRN 236  Severe acute respiratory syndro...
YP_803213    6765 LQVNEIVKyFGSWKAVTFAvnySSSETFLFCAgytg-veyNTSIVQng----yMSLLGGYRK 6821 White bream virus
P0C6V7       6746 IQLSQFTKyFSAVQFFTAGvntSSSEVFVVLKyk----lySEPIGEelcspniLSRIAAYRN 6803 Berne virus
AVM87336     6727 IDAESILPyFGSFTFVTFAvnySSSETYAFLKgymgepnyNTTLPAn-----iMGQLGAYRQ 6783 Chinook salmon bafinivirus
AVM87568     2976 TNLGQLTRyFSSWTRIFCEvknQSSETWIVFAhkl---lsPRQVPLed----tWGIMQCLER 3030 Hainan hebius popei arterivirus
YP_009505581 7069 VDVPKIVKyFGNWKLITFAinySSSETFLWCSgyta-tdyNESVVQnd----iMSLLGGYRQ 7125 Fathead minnow nidovirus
QEG08237     2607 DVVFQLCKhCSWWGIVFTRvnaSSSEYFLIGTnynp-hvfNETLPDfs---svRAGYAWFRN 2664 Pacific salmon nidovirus
P0C6V8       6622 IQLSKVTKyFAAVHFFTAGvntSSSEVFVVLKyk----lhSEPIGEelcspniLRRIAAYRN 6679 Breda virus serotype 1

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