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Conserved domains on  [gi|1796318603|ref|YP_009724395|]
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ORF7a protein [Severe acute respiratory syndrome coronavirus 2]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ORF7a_SARS-CoV-2-like cd21684
Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) structural accessory protein ...
 1-121 3.78e-74

Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) structural accessory protein ORF7a and a bat coronavirus (BatCoV RaTG13) from related betacoronaviruses in the subgenera Sarbecovirus (B lineage); This group contains the structural accessory protein ORF7a, also called NS7a, of Severe Acute Respiratory Syndrome Coronaviruses (SARS-CoV) from betacoronavirus subgenera Sarbecovirus (lineage B), including SARS-CoV-2, also known as 2019-nCoV, and a bat coronavirus (BatCoV RaTG13), which was previously detected in Rhinolophus affinis from China's Yunnan province. ORF7a/NS7a from betacoronavirus in the subgenera Sarbecovirus (B lineage) are not related to NS7a proteins from other coronavirus lineages. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the ORF1ab (a large polyprotein known as replicase/protease); all required to produce a structurally complete viral particle. In addition, SARS-CoV contains a number of open reading frames that code for a total of eight accessory proteins, namely ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b. These ORFs are specific for SARS-CoV and do not show significant homology to accessory proteins of other coronaviruses. Structurally, ORF7a possesses a distinctive immunoglobulin (Ig)-like domain which is related to extracellular metazoan Ig domains that are involved in adhesion, such as ICAM; it also contains a 15-aa signal peptide sequence at its N terminus, an 81-aa luminal domain, a 21-aa transmembrane domain, and a short C-terminal tail. Coexpression of SARS-CoV ORF7a with S, M, N, and E proteins resulted in production of virus-like particles (VLPs) carrying ORF7a protein, indicating that ORF7a is a viral structural protein. Expression studies of ORF7a have shown that biological functions include induction of apoptosis through a caspase-dependent pathway, activation of the p38 mitogen-activated protein kinase signaling pathway, inhibition of host protein translation, and suppression of cell growth progression. These results collectively suggested that ORF7a protein may be involved in virus-host interactions.


:

Pssm-ID: 394935  Cd Length: 121  Bit Score: 216.04  E-value: 3.78e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1796318603   1 MKIILFLALITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRAR 80
Cdd:cd21684     1 MKIILFLVLITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRAR 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1796318603  81 SVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE 121
Cdd:cd21684    81 SVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE 121
 
Name Accession Description Interval E-value
ORF7a_SARS-CoV-2-like cd21684
Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) structural accessory protein ...
 1-121 3.78e-74

Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) structural accessory protein ORF7a and a bat coronavirus (BatCoV RaTG13) from related betacoronaviruses in the subgenera Sarbecovirus (B lineage); This group contains the structural accessory protein ORF7a, also called NS7a, of Severe Acute Respiratory Syndrome Coronaviruses (SARS-CoV) from betacoronavirus subgenera Sarbecovirus (lineage B), including SARS-CoV-2, also known as 2019-nCoV, and a bat coronavirus (BatCoV RaTG13), which was previously detected in Rhinolophus affinis from China's Yunnan province. ORF7a/NS7a from betacoronavirus in the subgenera Sarbecovirus (B lineage) are not related to NS7a proteins from other coronavirus lineages. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the ORF1ab (a large polyprotein known as replicase/protease); all required to produce a structurally complete viral particle. In addition, SARS-CoV contains a number of open reading frames that code for a total of eight accessory proteins, namely ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b. These ORFs are specific for SARS-CoV and do not show significant homology to accessory proteins of other coronaviruses. Structurally, ORF7a possesses a distinctive immunoglobulin (Ig)-like domain which is related to extracellular metazoan Ig domains that are involved in adhesion, such as ICAM; it also contains a 15-aa signal peptide sequence at its N terminus, an 81-aa luminal domain, a 21-aa transmembrane domain, and a short C-terminal tail. Coexpression of SARS-CoV ORF7a with S, M, N, and E proteins resulted in production of virus-like particles (VLPs) carrying ORF7a protein, indicating that ORF7a is a viral structural protein. Expression studies of ORF7a have shown that biological functions include induction of apoptosis through a caspase-dependent pathway, activation of the p38 mitogen-activated protein kinase signaling pathway, inhibition of host protein translation, and suppression of cell growth progression. These results collectively suggested that ORF7a protein may be involved in virus-host interactions.


Pssm-ID: 394935  Cd Length: 121  Bit Score: 216.04  E-value: 3.78e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1796318603   1 MKIILFLALITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRAR 80
Cdd:cd21684     1 MKIILFLVLITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRAR 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1796318603  81 SVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE 121
Cdd:cd21684    81 SVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE 121
bCoV_NS7A pfam08779
Betacoronavirus NS7A protein; This entry corresponds to the Human SARS virus NS7A protein ...
 16-121 1.52e-64

Betacoronavirus NS7A protein; This entry corresponds to the Human SARS virus NS7A protein (also known as 7a, accessory protein 7a, X4 and U122). The structure of this protein shows similarities to the immunoglobulin like fold and a known member of the immunoglobulin (Ig) domain superfamily, which suggests a binding activity to integrin I domains. In SARS-CoV-infected cells, the NS7A protein is expressed and retained intra-cellularly within the Golgi network. NS7A has been implicated to function during the replication cycle of SARS-CoV.


Pssm-ID: 462600  Cd Length: 107  Bit Score: 191.07  E-value: 1.52e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1796318603  16 ELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEV-Q 94
Cdd:pfam08779   1 ELYHYQECVRGTTVLLKEPCPSGTYEGNSPFHPLADNKFALTCTSTHFAFACADGTRHTYQLRARSVSPKLFIRQEEVqQ 80

                          90       100
                  ....*....|....*....|....*..
gi 1796318603  95 ELYSPIFLIVAAIVFITLCFTLKRKTE 121
Cdd:pfam08779  81 ELYSPLFLIVAALVFLILCFTIKRKTE 107
 
Name Accession Description Interval E-value
ORF7a_SARS-CoV-2-like cd21684
Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) structural accessory protein ...
 1-121 3.78e-74

Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) structural accessory protein ORF7a and a bat coronavirus (BatCoV RaTG13) from related betacoronaviruses in the subgenera Sarbecovirus (B lineage); This group contains the structural accessory protein ORF7a, also called NS7a, of Severe Acute Respiratory Syndrome Coronaviruses (SARS-CoV) from betacoronavirus subgenera Sarbecovirus (lineage B), including SARS-CoV-2, also known as 2019-nCoV, and a bat coronavirus (BatCoV RaTG13), which was previously detected in Rhinolophus affinis from China's Yunnan province. ORF7a/NS7a from betacoronavirus in the subgenera Sarbecovirus (B lineage) are not related to NS7a proteins from other coronavirus lineages. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the ORF1ab (a large polyprotein known as replicase/protease); all required to produce a structurally complete viral particle. In addition, SARS-CoV contains a number of open reading frames that code for a total of eight accessory proteins, namely ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b. These ORFs are specific for SARS-CoV and do not show significant homology to accessory proteins of other coronaviruses. Structurally, ORF7a possesses a distinctive immunoglobulin (Ig)-like domain which is related to extracellular metazoan Ig domains that are involved in adhesion, such as ICAM; it also contains a 15-aa signal peptide sequence at its N terminus, an 81-aa luminal domain, a 21-aa transmembrane domain, and a short C-terminal tail. Coexpression of SARS-CoV ORF7a with S, M, N, and E proteins resulted in production of virus-like particles (VLPs) carrying ORF7a protein, indicating that ORF7a is a viral structural protein. Expression studies of ORF7a have shown that biological functions include induction of apoptosis through a caspase-dependent pathway, activation of the p38 mitogen-activated protein kinase signaling pathway, inhibition of host protein translation, and suppression of cell growth progression. These results collectively suggested that ORF7a protein may be involved in virus-host interactions.


Pssm-ID: 394935  Cd Length: 121  Bit Score: 216.04  E-value: 3.78e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1796318603   1 MKIILFLALITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRAR 80
Cdd:cd21684     1 MKIILFLVLITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRAR 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1796318603  81 SVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE 121
Cdd:cd21684    81 SVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE 121
bCoV_NS7A pfam08779
Betacoronavirus NS7A protein; This entry corresponds to the Human SARS virus NS7A protein ...
 16-121 1.52e-64

Betacoronavirus NS7A protein; This entry corresponds to the Human SARS virus NS7A protein (also known as 7a, accessory protein 7a, X4 and U122). The structure of this protein shows similarities to the immunoglobulin like fold and a known member of the immunoglobulin (Ig) domain superfamily, which suggests a binding activity to integrin I domains. In SARS-CoV-infected cells, the NS7A protein is expressed and retained intra-cellularly within the Golgi network. NS7A has been implicated to function during the replication cycle of SARS-CoV.


Pssm-ID: 462600  Cd Length: 107  Bit Score: 191.07  E-value: 1.52e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1796318603  16 ELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEV-Q 94
Cdd:pfam08779   1 ELYHYQECVRGTTVLLKEPCPSGTYEGNSPFHPLADNKFALTCTSTHFAFACADGTRHTYQLRARSVSPKLFIRQEEVqQ 80

                          90       100
                  ....*....|....*....|....*..
gi 1796318603  95 ELYSPIFLIVAAIVFITLCFTLKRKTE 121
Cdd:pfam08779  81 ELYSPLFLIVAALVFLILCFTIKRKTE 107
ORF7a_SARS-CoV-like cd21663
Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) structural accessory protein ORF7a ...
 14-95 2.86e-57

Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) structural accessory protein ORF7a and similar proteins from related betacoronaviruses in the subgenera Sarbecovirus (B lineage); This family contains the structural accessory protein ORF7a, also called NS7a, of Severe Acute Respiratory Syndrome Coronaviruses (SARS-CoVs) from betacoronavirus subgenera Sarbecovirus (lineage B), including SARS-CoV-2, also known as 2019-nCoV, and a bat coronavirus (BatCoV RaTG13), which was previously detected in Rhinolophus affinis from China's Yunnan province, as well as SARS-related virus from Rhinolophus bats in Europe and Kenya. ORF7a/NS7a from betacoronavirus in the subgenera Sarbecovirus (lineage B) are not related to NS7a proteins from other coronavirus lineages. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the ORF1ab (a large polyprotein known as replicase/protease); all required to produce a structurally complete viral particle. In addition, SARS-CoV contains a number of open reading frames that code for a total of eight accessory proteins, namely ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b. These ORFs are specific for SARS-CoV and do not show significant homology to accessory proteins of other coronaviruses. Structurally, ORF7a possesses a distinctive immunoglobulin (Ig)-like domain which is related to extracellular metazoan Ig domains that are involved in adhesion, such as ICAM; it also contains a 15-amino acid signal peptide sequence at its N terminus, an 81-amino acid luminal domain, a 21-amino acid transmembrane domain, and a short C-terminal tail. Co-expression of SARS-CoV ORF7a with S, M, N and E proteins resulted in production of virus-like particles (VLPs) carrying ORF7a protein, indicating that ORF7a is a viral structural protein. Expression studies of ORF7a have shown that biological functions include induction of apoptosis through a caspase-dependent pathway, activation of the p38 mitogen-activated protein kinase signaling pathway, inhibition of host protein translation, and suppression of cell growth progression. These results collectively suggested that ORF7a protein may be involved in virus-host interactions.


Pssm-ID: 394934  Cd Length: 83  Bit Score: 172.08  E-value: 2.86e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1796318603  14 TCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEV 93
Cdd:cd21663     1 SAELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCTSTKFAFACQDGTRHTYQLRARSVSPKLFIRQEEV 80


                  ..
gi 1796318603  94 QE 95
Cdd:cd21663    81 QQ 82
ORF7a_SARS-CoV-like cd21685
Severe Acute Respiratory Syndrome coronavirus (SARS-CoV-2) structural accessory protein ORF7a ...
 14-95 1.90e-54

Severe Acute Respiratory Syndrome coronavirus (SARS-CoV-2) structural accessory protein ORF7a and similar proteins from betacoronaviruses in the subgenera Sarbecovirus (B lineage); This group contains the structural accessory protein ORF7a, also called NS7a, of Severe Acute Respiratory Syndrome Coronaviruses (SARS-CoVs) from betacoronavirus subgenera Sarbecovirus (lineage B). ORF7a/NS7a from betacoronavirus in the subgenera Sarbecovirus (B lineage) are not related to NS7a proteins from other coronavirus lineages. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the ORF1ab (a large polyprotein known as replicase/protease); all required to produce a structurally complete viral particle. In addition, SARS-CoV contains a number of open reading frames that code for a total of eight accessory proteins, namely ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b. These ORFs are specific for SARS-CoV and do not show significant homology to accessory proteins of other coronaviruses. Structurally, ORF7a possesses a distinctive immunoglobulin (Ig)-like domain which is related to extracellular metazoan Ig domains that are involved in adhesion, such as ICAM; it also contains a 15-aa signal peptide sequence at its N terminus, an 81-aa luminal domain, a 21-aa transmembrane domain, and a short C-terminal tail. Coexpression of SARS-CoV ORF7a with S, M, N, and E proteins resulted in production of virus-like particles (VLPs) carrying ORF7a protein, indicating that ORF7a is a viral structural protein. Expression studies of ORF7a have shown that biological functions include induction of apoptosis through a caspase-dependent pathway, activation of the p38 mitogen-activated protein kinase signaling pathway, inhibition of host protein translation, and suppression of cell growth progression. These results collectively suggested that ORF7a protein may be involved in virus-host interactions.


Pssm-ID: 394936  Cd Length: 83  Bit Score: 164.87  E-value: 1.90e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1796318603  14 TCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEV 93
Cdd:cd21685     1 SCELYHYQECVRGTTVLLKEPCPSGTYEGNSPFHPLADNKFALTCTSTHFAFACADGTRHTYQLRARSVSPKLFIRQEEV 80


                  ..
gi 1796318603  94 QE 95
Cdd:cd21685    81 HQ 82
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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